About the HTS Facility

The recent boon in high-throughput sequencing technologies has offered enormous potential to applications in genome sequencing, transcriptome profiling, epigenetics, metagenomics, discovery of non-coding RNAs and protein binding sites. A primary mission of PGFI is to lead the development of new genomics technologies at the University of Pennsylvania. In response to the ever growing demand for high-throughput sequencing at Penn, PGFI’s HTS facility was established.

In this facility, investigators have access to different types of HTS technology: Illumina’s HiSeq and Applied Biosystem’s SOLiD4 and 5500xl. With two HiSeqs, the facility can provide the latest innovation from Illumina featuring up to 3 billion paired-end reads (up to 100x100 bp reads) per flow cell, 8 lanes per flow cell and 2 flow cells per machine. With the SOLiD4, the facility can provide up to 1.4 billion reads per slide, 50 x 25 bp paired-end reads, up to 8 sectors per slide and 2 flow cells per machine. With the 5500xl, the facility can provide up to 180 Gb or more than 2.8 B reads per run. The 5500xl offers 75 bp fragment runs, 75x35 bp paired-end runs and up to 60 bp x 60 bp mate paired runs with the added bonus of pay-as-you-go sequencing. All platforms offer multiplexing capability. Currently Illumina offers 48 barcodes and Life Technologies offers 96 barcodes. The SOLiD 4 system provides greater than 99.94% accuracy due to 2-base encoding. The 5500xl offers up to 99.99% accuracy utilizing 2-base encoding and Exact Call Chemistry.

• About the HTS Facility Technologies

• HTS Price List (updated February 2013)

• Submit an HTS Request
  • HTS Application Form (must be submitted first)
  • PathBio: web application for submitting work requests and monitoring job progress (once above HTS Application has been accepted)

• HTS Policies

• HTS FAQs

• Additional Resources and Useful Links

HTS Contacts

Project Manager
Jeanne Geskes
304D Carolyn Lynch Laboratory
PGFI
University of Pennsylvania
Philadelphia, PA 19104
215-573-4642
F 215-746-6258
jeanneg(at)sas.upenn.edu