Single Cell Analysis Symposium

March 12, 2014 @ the University of Pennsylvania

Hold the date for this all-day symposium. Speakers include Nancy Allbritton, Helen Blau, Chi Dang, Jim Eberwine, Junhyong Kim, Christopher Murray, Garry Nolan, Arjun Raj and Sherman Weissman.

More details and registration here for this free event.



 

The next frontier in the genome sciences will be developing technology that captures novel quantitative biological measurements ... more about genomics

High-Throughput Sequencing Facility

About the HTS Facility

As of November 2013, the PGFI HTS Facility is no longer accepting job requests. The Functional Genomics Core in the Perelman School of Medicine may be able to help you.

The recent boon in high-throughput sequencing technologies has offered enormous potential to applications in genome sequencing, transcriptome profiling, epigenetics, metagenomics, discovery of non-coding RNAs and protein binding sites. A primary mission of PGFI is to lead the development of new genomics technologies at the University of Pennsylvania. In response to the ever growing demand for high-throughput sequencing at Penn, PGFI’s HTS facility was established.

In this facility, investigators have access to different types of HTS technology: Illumina’s HiSeq and Applied Biosystem’s SOLiD4 and 5500xl. With two HiSeqs, the facility can provide the latest innovation from Illumina featuring up to 3 billion paired-end reads (up to 100x100 bp reads) per flow cell, 8 lanes per flow cell and 2 flow cells per machine. With the SOLiD4, the facility can provide up to 1.4 billion reads per slide, 50 x 25 bp paired-end reads, up to 8 sectors per slide and 2 flow cells per machine. With the 5500xl, the facility can provide up to 180 Gb or more than 2.8 B reads per run. The 5500xl offers 75 bp fragment runs, 75x35 bp paired-end runs and up to 60 bp x 60 bp mate paired runs with the added bonus of pay-as-you-go sequencing. All platforms offer multiplexing capability. Currently Illumina offers 48 barcodes and Life Technologies offers 96 barcodes. The SOLiD 4 system provides greater than 99.94% accuracy due to 2-base encoding. The 5500xl offers up to 99.99% accuracy utilizing 2-base encoding and Exact Call Chemistry.

  • HTS Price List (updated February 2013)
  • Submit an HTS Request
    • HTS Application Form (must be submitted first)
    • PathBio: web application for submitting work requests and monitoring job progress (once above HTS Application has been accepted)
  • Additional Resources and Useful Links

 

PGFI Announcements

The PGFI Fall 2013 Newsletter can be viewed here.

Educational Facility Professional Development Workshops can be applied for here.

 

 

Based at the University of Pennsylvania, Penn Genome Frontiers Institute (PGFI) is a university-wide institute dedicated to the advancement of the interdisciplinary field of genomics research. PGFI fosters collaborations and scientific exchange across biology, veterinary medicine, pharmacology, medicine, genetics, microbiology, engineering, physics, chemistry and psychology. Our mission...